Virus for PacBio SMRT RNA-Seq Library Constion Kit

九游娱乐(NineGameSports)同汇
Fri, 18 Oct 2019 13:14:32 +0000
三代转录组产品, 产品, SP0041S, 三代转录组

试剂盒组分：

1. 试剂盒1

目的 | 试剂 | PacBio | Nanopore
SP0021 | SN0021
M/10次 | L/20次 | M/10次 | L/20次

反

转

录

1

●SUPERSWITCH? 3RRT Oligonucleotide

12μL

24 μL

6μL

12μL

2 | ●dNTP Mix (10 mM) | 40 μL | 80 μL | 20 μL | 40 μL
3 | ●Deionized H₂O | 100 μL | 100 μL | 100 μL | 100 μL
4 | ●5X First-Strand Buffer (RNase-Free) | 60 μL | 120 μL | 30 μL | 60 μL
5 | ●SUPERSWITCH? SolutionⅠ | 10 μL | 20 μL | 5 μL | 10 μL
6 | ●SUPERSWITCH? SolutionⅡ | 10 μL | 20 μL | 5 μL | 10 μL
7 | ●RNase Inhibitor (40 U/μL) | 12 μL | 25 μL | 6 μL | 12 μL
8 | ●SUPERSWITCH? Reverse Transcriptase (200 U/μL) | 12 μL | 25 μL | 6 μL | 12 μL
9 | ●SUPERSWITCH? 5RRT Oligonucleotide | 12 μL | 24 μL | 6 μL | 12 μL
PCR | 10 | ●5AP PCR Primer (12 μM) | 100 μL | 200 μL | 100 μL | 200 μL
11 | ●3AP PCR Primer (12 μM) | 100 μL | 200 μL | 100 μL | 200 μL
12 | ●Marathon DNA Polymerase（1U/μL） | 24μL | 48μL | 24 μL | 48 μL
13 | ●2×PCR Buffer | 765μL | 1530 μL | 765μL | 1530 μL
14 | ●2.5 mM dNTP | 160μL | 320 μL | 200 μL | 400 μL

1. 试剂盒2：Tiosbio? 200bp Cleaner

目的 | 试剂 | PacBio | Nanopore
M/30次 | L/60次 | M/30次 | L/60次

PCR

产物

纯化

1

○Buffer P5

7.5 mL

15 mL

15 mL

30 mL

2 | ○Wash Buffer | 3.25 mL | 6.5 mL | 6.5 mL | 13 mL